摘要
目的测定西安地区肠杆菌科细菌中的超广谱β-内酰胺酶(extended-spectrum β-lactamases,ESBL)的基因型。方法在西安市的5所医院中随机选取产ESBL的肠杆菌科细菌25株(共125株),采用PCR方法特异性扩增TEM、SHV和CTX-M型酶的基因片段。结果在125株产ESBL菌中,有33株扩增出TEM型酶基因片段;有27株扩增出特异性SHV型酶基因片段;有49株扩增出特异性的CTX-M型酶基因片段;共有22株细菌同时扩增出两种以上的酶基因片段。结论在西安地区的ESBL肠杆菌科细菌中CTX-M型酶较为流行。
Objective To study the genotypes of ESBL-producing Enterobacteriaceae in Xi＇an city. Methods Totally 125 ESBL-producing Enterobacteriaceae strains were randomly selected from five hospitals of Xi＇an City, and TEM-type, SHV-type and CTX-M-type ESBL genes were amplified by PCR. Results Among the 125 ESBL-producing Enterobacteriaceae strains, TEM-type ESBL genes were amplified from 33 strains, SHV-type ESBL genes were amplified from 26 strains, CTX-M-type ESBL genes were amplified from 49 strains, and two or more type ESBL genes were amplified from 22 strains. Conclusion CTX-M-type ESBL are prevalent in ESBL- producing Enterbacteriaceae in Xi＇an City.
出处
《西安交通大学学报（医学版）》
CAS
CSCD
北大核心
2008年第4期443-445,共3页
Journal of Xi’an Jiaotong University（Medical Sciences）
基金
陕西省科技攻关基金资助项目(No.2002K10-G5)
