丰香草莓叶片的植株再生研究

Plant Regeneration of Strawberry Leaves of "Fengxiang"

[目的]为采用叶片组织培养技术进行大规模生产草莓种苗提供依据。[方法]以丰香草莓叶片为外植体,用4种不同的培养基诱导其愈伤组织,并对愈伤组织进行扩繁,筛选最佳的诱导培养基。[结果]MS+6-BA 0.5 mg/L+NAA 0.1 mg/L培养基最快产生愈伤组织,愈伤组织诱导率、生根率、不定芽诱导率均最高,分别为69.4%、10%、11.67%,且愈伤组织体积较大,生根总数最多,不定芽生长茂盛,叶片多而大,最终获得具根系可移植室外的完整植株543株,增殖扩繁倍数达50,增殖效果极好。MS+6-BA 1.0 mg/L+NAA 0.1mg/L培养基的愈伤组织诱导率为63.8%,且愈伤组织体积最大。[结论]草莓叶片诱导愈伤组织、不定芽并最终获得大量再生植株的最佳培养基是:MS+6-BA 0.5 mg/L+NAA 0.1 mg/L。

[ Objective ] The purpose was to provide the basis for mass production of strawberry seedling by adopting of leaf tissue culture technology. [ Method ] With ＂Fengxiang＂ strawberry leaves as explant, its callus was induced with 4 kinds of different culture media, and expanding propagation was performed to on the callus, and the best induction medium was screened. [ Result] The medium of MS ＋ 6-BA 0.5 mg/L ＋ NAA 0.1 mg/L generated callus the fastest, on which, the induction rate of callus, rooting rate and induction rate of adventitious bud were all the highest, up to 69.4%, 10.00% and 11. 67% resp. , and the callus volume was bigger, the total number of rooting was the most, the adventitious bud grown flourishingly and leaves were more and big, eventually 543 complete plantlets with roots that could be transplanted to outdoors were acquired, the multiple of proliferation and expanding propagation was up to 50 and the proliferation effect was extremely good. On the medium of MS ＋ 6-BA 1.0 mg/L ＋ NAA 0.1 rag/L, the callus induction of was 63.8% and the volume of callus was the largest. [ Conclusion] The optimal medium for strawberry leaves on inducing callus, adventitious bud and acquiring plenty of regenerating plantlet eventually was： MS ＋6-BA 0.5 mg/L ＋ NAA 0.1 mg/L.