冷休克诱导黄河鲇(Silurus asotus)二倍体雌核发育

Induction on Gynogenesis of Cold Shock in Silurus astus

[目的]通过冷休克法诱导黄河鲇二倍体雌核发育。[方法]在水温23.5±0.5℃的条件下,用紫外线照射黄河鲇(Silurus asotus)精子,然后进行进行人工受精,设置不同的冷休克起始时间和持续处理时间,观察黄河鲇二倍体雌核发育。[结果]在紫外线照射15min、冷休克起始时间为受精后5 min、持续处理40 min的条件下得到的二倍体黄河鲇成活率最高,达8.5%。[结论]该研究为培育优良黄河鲇新品种奠定了基础,为深入研究雌核发育机理积累了原始资料。

[ Objective] The cold shock method was used to induce diploid gynogenesis of Silurus astus. [ Method] Silurus astus sperms were irradi ated by ultraviolet then conducted fertilization. Different cold shock starting time and duration were set to observe diploid gynogenesis of Silurus astus. [ Result] Under such inducing condition that ultraviolet irradiated 15 min ,cold shock started 5 min after fertilization and cold shock duartion was 40 rain,the survival rate of diploid Silurus astus was highest （ 8.5 % ） [ Conclusion ] The experiment laid foundation for culturing new excellent Silurus astus varieties and accumulated original files for further study of gynogenesisdevelopment mechanism,