磁场对乌药试管苗生长的生物学效应研究

Study on the Biological Effect of Magnetic Field on Growth of Lindera aggregata (Sims) kosterm Plantlet in vitro

[目的]为磁处理在乌药规模化育苗中的应用提供理论依据。[方法]以乌药试管苗为对象,在6、12、17、27 mT 4个磁感应强度(一组)分别与5、10、15 h 3个处理时间的组合条件下,研究磁场对乌药试管苗生长的生物学影响,筛选最佳的磁处理条件。[结果]不同磁处理条件对乌药试管苗生长的生物学效应不同。27 mT/15 h对苗高,17 mT/15 h与27 mT/15 h对不定芽,12 mT/15 h、17 mT/15 h与27 mT/15 h对乌药试管苗重量均具有抑制作用;其他磁处理条件对乌药试管苗的苗高、不定芽、重量等均有不同程度的促进作用。[结论]4个磁感应强度(6、12、17、27 mT)与10 h的组合对乌药试管苗生长(苗高、不定芽、苗重)都具有正生物学效应。17 mT/10 h对苗高、6 mT/10 h与6 mT/15 h对不定芽、6 mT/10 h对乌药试管苗重量均具有明显的正生物学效应。

[ Objective ] This paper was conducted to offer theoretical references for the application of magnetic treatment in growth of Lindera aggregata （Sims） kosterm plantlet in vitro. [ Method] Taking L. aggregata plantlet in vitro as test material, the effects of magnetic field on the growth of L. aggregata plantlet in vitro were studied under four magnetic induction densities （6, 12, 17 and 27 mT） and three treatments （5, 10 and 15 h）. The optimum magnetic treatment conditions were selected. [ Result] Different magnetic treatment conditions had different biological effects on the growth of L. aggregata plantlet in vitro. There were inhibitory effect for 27 mT/15 h on plantlet height, 17 mT/15 h and 27 mT/15 h on adventitious buds, 12 mT/15 h, 17 mT/15 h and 27 mT/15 h on the weight of L. aggregata plantlet in vitro. Other magnetic treatments had different promoting effects on the seedling height, adventitious bud and weight of L. aggregata plantlet in vitro. [ Conclusion] The combination of 6, 12, 17 and 27 naT with 10 h had plus biological effect on the growth of L. aggregata plantlet in vitro. There were significant plus biological effect for 17 mT/10 h on the height, 6 mT/10 h and 6 mT/15 h on adventitious bud and 6 mT/10 h on the weight of L. aggregata plantlet in vitro.