MMP1 mRNA在口腔鳞癌与正常黏膜中的表达及临床意义

Expression of MMP1 mRNA in oral squamous cell carcinoma and paired normal tissues

目的探讨基质金属蛋白酶1(MMP1)mRNA在口腔鳞状细胞癌(OSCC)及配对正常口腔黏膜组织中的表达及临床意义。方法用RT-PCR方法检测30例OSCC患者肿瘤组织及其配对的正常口腔粘膜中MMP1mRNA的表达;所得数据采用t-test检验方法进行统计学处理。结果RT-PCR检测结果表明,MMP1mRNA在OSCC和配对正常粘膜的表达水平分别为4.06±0.52和1.24±0.17,上调3.26倍(P<0.0001)。在30例OSCC标本中,MMP1mRNA表达在病理Ⅱ/Ⅲ级OSCC中的表达(4.31±0.68)高于Ⅰ级OSCC的表达(3.87±0.57)(P>0.05);在临床Ⅲ/Ⅳ期OSCC患者标本中的表达水平(4.18±0.67)高于Ⅰ/Ⅱ期OSCC的表达水平(3.65±0.53)(P>0.05);在颈淋巴结阳性OSCC患者的表达水平(4.32±0.71)高于颈淋巴结阴性OSCC患者的表达水平(3.91±0.51)(P>0.05)。结论MMP1可能在OSCC的侵袭过程中起重要作用,可能是OSCC诊断、治疗及预后判断的一个基因靶标;MMP1在OSCC分类诊断中有应用价值。

Objective To investigate the mRNA expression of matrix metalloproteinase 1 （MMP1） gene in oral squamous cell carcinoma （OSCC） and the paired normal tissues. Methods The differential expression of MMP1 mRNA between 30 OSCC and paired normal tissues were detected with reverse transcription-PCR （RT-PCR）. Results The relative expression level of MMP1 mRNA in the OSCC tissues showed a 3.26-fold increase in comparison with that in the paired normal tissues （4.06±0.52 vs 1.24±0.17, P〈0.0001）. In the 30 OSCC tissues, the relative expression level of MMP1 mRNA was higher in histological grade II/III tissues （4.31±0.68） than in grade I （3.87±0.57） tissues, higher in OSCC in advanced stages （III/IV） than in tumors in early stages （I/II） （4.18±0.67 vs 3.65±0.53）, and also higher in OSCC with cervical lymph node invasion than in those without cervical lymph node invasion （4.32±0.71 vs 3.91±0.51）, but these differences were not statistically significant （P〉0.05）. Conclusion MMP1 gene may play a role in local invasion of OSCC, and can serve as a potential biomarker molecule for diagnosis, treatment and prognostic evaluation of OSCC, with also clinical value for OSCC classification.