半叶马尾藻多糖对抑癌基因p53和Rb的影响

Effects of Sargassum confusum polysaccharide on the expression of p53 and Rb genes in mouse sarcoma S180 cells

目的研究半叶马尾藻多糖(SP)对抑癌基因p53和Rb的影响。方法称重法检测SP对小鼠S180肉瘤的抑瘤率;RT-PCR分析抑癌基因p53和Rb中mRNA的含量;Western-blotting检测p53和Rb中蛋白质的表达水平。结果将浓度分别为50、100和200mg/kg的SP经腹腔注射入S180肉瘤小鼠体内,连续10d,抑瘤率分别为30.5%、47.6%和63.5%。SP可上调抑癌基因p53和Rb中mRNA的表达以及增加p53和Rb中蛋白质含量。结论SP对小鼠S180肉瘤具有一定的体内抑制作用,并上调抑癌基因的表达。

Objective To investigate effects of Sargassum confusum polysaccharide （SP） on the expressions of p53 and Rb genes. Methods The mice bearing S180 sarcoma were treated with abdominal SP injection at the daily dose of 50, 100 or 200 mg/kg for 10 consecutive days, The mice injected with 0.85% saline served as the negative control group and those with 5-flurouracil （5-FU） injection as the positive control group. The tumor weight was measured to calculate the tumor inhibition rate. RT-PCR and Western blotting were used to detect the expression levels of p53 and Rb genes at the mRNA and protein levels, respectively. Results The 10-day SP treatment at 50, 100 and 200 mg/kg resulted in tumor growth inhibition rates of 30.5%, 47.6% and 63.5%, respectively. SP treatment upregulated the mRNA expressions and increased the protein levels of p53 and Rb in the tumors. Conclusion SP has inhibitory effect against S180 sarcoma in vivo and can increase the expression levels of p53 and Rb genes at both the mRNA and protein levels.