摘要
目的探讨原因不明复发性流产(URSA)患者外周血及蜕膜组织中CD4+cD夹调节性T(Tr)细胞比例的变化。方法采用双荧光标记流式细胞分析技术检测25例URSA患者(流产组)、34例正常早孕妇女(正常妊娠组)和22例正常非孕妇女(正常非孕组)外周血及蜕膜组织中CD4^+CD25^+Tr细胞的比例。结果(1)流产组和正常妊娠组妇女外周血CD4^+CD25^bright T细胞的比例[分别为(1.55±0.77)%、(2.65±1.10)%]均显著高于正常非孕组妇女[(0.39±0.14)%],分别比较,差异均有统计学意义(P〈0.05);流产组妇女外周血CD4^+CD25^bright T细胞的比例显著低于正常妊娠组妇女,两组比较,差异有统计学意义(P〈0.05)。(2)流产组妇女蜕膜组织中CD4^+CD25^bright T细胞比例[(0.59±0.23)%]显著低于正常妊娠组妇女[(1.24±0.55)%],两组比较,差异有统计学意义(P〈0.01)。流产组妇女蜕膜组织中CD4^+CD25^bright T细胞比例[(4.23±1.52)%]与正常妊娠组[(3.75±1.88)%]比较,差异无统计学意义(P〉0.05)。(3)正常妊娠组妇女蜕膜组织中CD4^+CD25^bright T细胞占CD4^+ T细胞的比例(CD4^+CD25^bright/CD4^+)为(13.10±10.25)%,显著高于外周血[(5.59±2.62)%],两者比较,差异有统计学意义(P〈0.05);流产组患者蜕膜组织中CD4^+CD25^bright,/CD4^+比例[(5.16±2.83)%]与外周血[(4.64±2.07)%]比较,差异无统计学意义(P〉0.05)。结论CD4^+CD25^bright Tr细胞数量在早孕期显著升高,参与了正常妊娠的维持,有可能是调控母.胎界面局部免疫耐受形成的一个重要因素;CD4^+CD25^bright Tr细胞数量的减少可能与URSA的发生有关。
Objective To study the changes in the percentage of CD4^+CD25^+ regulatory T (Tr) cells in peripheral blood and deciduas in unexplained recurrent spontaneous abortion (URSA) patients, normal non-pregnant and pregnant women respectively. Methods The percentage of CD4^+CD25^+ Tr cells in deciduas and peripheral blood from 25 URSA patients, 22 normal non-pregnant (NNP) women, and 34 normal early pregnant (NP) women were measured by double-staining followed by flow cytometric analysis. Results + bright ( 1 ) The percentage of CD4^+CD25^bright T cells in peripheral blood in both URSA and NP [ ( 1.55 ±0. 77 ) %, (2.65 ± 1.10)%, respectively] women were increased significantly than that in NNP women [ (0. 39 ± 0. 14) %, P 〈 0. 05 ] . The percentage of CD4^+CD25^bright T cells in peripheral blood in URSA women was significantly lower than that in NP women ( P 〈 0. 05 ). ( 2 ) The percentage of CD4^+CD25^bright T cells in decidua in URSA women was significantly lower than that in NP women [ ( 0. 59 ± 0. 23 ) %, ( 1.24±0. 55) %, respectively, P 〈 0. 01 ]. There was no significant difference in the percentage of CD4^+CD25^bright T cells in decidua between URSA women and NP women [ (4. 23 ± 1.52) %, (3.75 ± 1.88) %, respectively, P〉0.05]. (3) The proportion of CD4^+CD25^bright, CD25 /CD4 cells in deciduas was significantly higher than that in peripheral blood in NP women [ ( 13.10 ± 10. 25 ) % , ( 5.59 ± 2. 62 ) % , respectively, P 〈 0. 05 ] . However, a significant difference in the proportion of CD4^+CD25^bright/CD4 between decidua and peripheral blood was not found in URSA patients [ ( 5.16 ± 2. 83 ) %, ( 4. 64 ± 2.07 ) % , respectively, P 〉 0. 05 ) ]. Conclusions The number of CD4^+CD25^+ Tr cells is increased in normal pregnancy and decreased in URSA. Therefore,CD4^+CD25^+ Tr cells may play an important role in maintaining maternal-fetal tolerance and may be involved in the pathogenesis of URSA.
出处
《中华妇产科杂志》
CAS
CSCD
北大核心
2008年第8期602-605,共4页
Chinese Journal of Obstetrics and Gynecology
基金
国家自然科学基金(30530740)