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HPLC-DAD-ESI-MS技术分离鉴定啤酒中异α-酸顺/反异构体

Investigation of Separation and Identification of cis/trans Stereoisomers of Iso-α-acid in Beer by HPLC-DAD-ESI-MS
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摘要 建立了1种采用高效液相法测定啤酒中异α-酸顺/反异构体的方法。样品通过异辛烷液液萃取获得,然后使用Purospher STAR RP_(18)(4.6 mm×250 mm,5μm)色谱柱,在甲醇-乙酸铵缓冲液作为流动相条件下等梯度分离,分离在50 min内完成。依据ESI-MS的选择性,使用高效液相色谱-二极管阵列检测器-电喷雾质谱联用(HPLC-DAD-ESI-MS)操作区分各化合物。根据保留时间、UV和MS图谱鉴定各目的蜂。该方法准确可靠,最低检测限0.21 mg/L。 An analytical method of cis/trans stereoisomers of iso-α-acid in beer was established. The sample was obtained by liquid/liquid extraction with iso-octane. Then aim compounds was separated by Purospher STAR RP18 （4.6 mm×250 mm, 5 μm） under an isocratic elution system of methanol-ammonium acetic buffer and completed within 50 minutes. The HPLC-diode array detector-electrospray ionization-mass spectroscopy （HPLC-DAD-ESI-MS） was used to differentiate these compounds according to the selectivity of the ESI-MS. The aim peaks were identified by the retention time, UV and mass spectra. The method is sensitive and accurate with a detection limit of iso-α-acids at 0.21 mg/L.
作者 宗明平 陶冠军 李崎 顾国贤
机构地区 江南大学教育部工业生物技术重点实验室
出处 《食品与发酵工业》 CAS CSCD 北大核心 2008年第7期127-131,共5页 Food and Fermentation Industries
基金 国家"十一五"重大科技支撑专项(No.2007BAK36801)
关键词 啤酒 异α-酸顺/反异构体 HPLC-DAD-ESI-MS beer, cis/trans stereoisomers of iso-α-acid, HPLC-DAD-ESI-MS
分类号 TS262.5 [轻工技术与工程—发酵工程]
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参考文献11

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食品与发酵工业
2008年 第7期

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