仙人掌SOD提取条件及提取过程活性稳定性研究

Study on Extraction Condition of Superoxide Dismutase from Cactu and Extracts Stability

以新鲜仙人掌为材料,采取直接打浆浸提的方式,提取仙人掌SOD。中性偏碱的缓冲液在30～35℃浸提效果较好。仙人掌SOD在60℃以下稳定性较强,可以采用55～60℃加热10～15min处理去除部分杂蛋白。V(二氮甲烷):V(乙醇)=2:1的比例处理SOD粗提液,也有利于去除杂蛋白,提高SOD比活性。仙人掌SOD在酸性条件下对紫外光敏感,碱性条件下对日光敏感,在pH 6～8对日光和紫外光都较稳定。

Superoxide dismutase（SOD） was extracted from milled fresh cactu. Extraction with buffer of slightly alkaline at 30-35℃ was preferable. Cactu SOD was stable when the precessing temperature was lower than 60℃. Heating 10-15min at 55-60℃ could be used for removing other mixed protein. Teating crude solution containing SOD with chloroform and alcohol of 2 ： 1 was also a prefer method for the elimination of other mixed protein, which could increase the activity per unit protein. Cactu SOD is sensitive to ultraviolet radiation in acidity condition and to daylight on alkaline solution. When the pH value of SOD solution was within 6-8, its activity was relatively stable to either ultraviolet radiation or daylight.