HPLC法测定乙肝清胶囊中槲皮素的含量

Determination of Quercetin in Yi Gan Qing Capsule by HPLC

[目的]建立乙肝清胶囊中槲皮素含量的HPLC测定方法。[方法]采用高效液相色谱法,色谱条件为：色谱柱,Phenomenex Luna C18柱（4.6mm×250mm,5μm）;流动相,甲醇-0.4%磷酸（50∶50）;流速,1ml/min;检测波长360nm;柱温30℃。[结果]槲皮素在0.054～1.08μg范围内与峰面积有良好的线性关系,相关系数r=0.99988,平均回收率99.15%,RSD1.42%（n=6）。[结论]该方法简便、准确、可靠,可作为乙肝清胶囊中槲皮素含量的测定方法。

The research aimed to establish the determination method of quercetin in Yi Gan Qing capsule by HPLC. [ Method] HPLC was used for determination, the chromatographic conditions were as follows： Phenomenex hma C18 column （4.6 mm× 250 mm, 5 μm）, methanol-0.4% H3PO4（50：50） as mobile phase, the detection wavelength of 360 nm, the flow rate of 1 ml/min and the column temperature of 30 %. [Result] Quercetin had good linear relationship with the peak area within the range of 0.054 - 1.08 pg, the correlation coefficient （r） was 0.999 88, the average recovery was 99.15% with RSD of 1.42% （ n =6）. [ Conclusion] This determination method is simple, accurate and reliable, so it could be used as the determination method of quercetin content in Yi Gan Qing capsule.