裸鼠结肠癌肝转移模型的建立

THE ESTABLISHMENT OF MODELS FOR XOLON CANCER LIVER METASTASIS

目的探讨建立裸鼠结肠癌肝转移模型的方法。方法45只裸鼠随机分为三组,分别用门静脉注射法,脾脏注射法和肝脏注射法建立结肠癌肝转移模型,并比较三种肝转移瘤模型在结肠癌肝脏转移率和自然生存期。结果门静脉注射组,脾脏注射组和肝脏注射组肝脏转移率分别为100%(15/15),60%(9/15),100%(15/15);自然生存期分别为41.47±2.35 d,o42.69±2.13 d,41.31±2.55 d。结论门静脉注射法建立裸鼠结肠癌肝转移模型是一种简便、有效的方法。

Objective To explore the methods to establish models for colon cancer liver metastasis. Methods Forty-five nude mice were diveded into 3 troups randomly, which were the portal vein injection group, spleen injection group and liver injection group; and there were 15 nude mice in each group, colon cancer liver metastasis models were made by portal vein injection, spleen injection and liver injection respectively, the metastasis rate and the life span was compared among the three methods. Results the metastasis rate for each group was 100% （15/15） ,60% （9/15） and 100% （ 15/15 ） respectively, and the life span was 41.47 ± 2. 35 d, 42. 69 ±2. 13 d and 41.31± 2.55 d accordingly. Conclusion Portal vein injection is a convenient and effective methods to establish models of colon cancer liver metastasis.