九种植物组织培养及其快速无性繁殖

Effect of Sulphurous Acid on Membrane Lipid Peroxidation in Wheat Seedlings and the Protective Function of Phosphate Buffer

以兰花花瓣、菊花、月季茎尖，桔梗、四季樱草、矮牵牛片、人参根、水稻花药及红松成熟胚为材料，材料经选择、消毒后，分别接种在各自培养基上（详见表１，２）．外植体经初次培养，继代培养，分化培养或生根培养，兰花由原球茎分化再生植株；菊花、月季通过丛生芽和不定根的诱导分化成再生植株，而桔梗、四季樱草、矮牵牛由愈伤组织分化不定芽（或胚状体），然后，形成再生植株；水稻由愈伤组织形成胚状体，通过胚状体再分化芽和根形成再生植株．

When the wheat seedlings were treated with lower concentration sulphurors acid (2mmol/L), the activities of superoxide dismuiase (SOD) in seedlings increased, while the levels of malondialdehyde (MDA) and electrolytic leakages were reduced. Sulphurous acids of higher concentration (>20mmol/L) could injure wheat seedlings. After a higher concentration sulphurous acids treatment, the SOD activities in seedlings were found to be reduced. while MDA contents and electrolytic leakage were found to be increased. Phosphate buffer could induce sulphourous acid s harmful action. It could induce the SOD activities of wheat seedlings, steadys membrane structure and protect plasma membrane. In this test SOD activities of wheat seedlings showed minus inter relation with the MDA contents and electrolytic leakages.