弓斑东方鱼屯同工酶生化表现型的初步研究

Effectes of IFN-γ on Corpus Luteum in False Pregnent Rat

利用聚丙烯酰胺凝胶垂直平板电泳方法，对弓斑东方（ｆｕｇｕｏｃｅｌａｔｕｓ）的５种同工酶ＥＳＴ、ＬＤＨ、ＳＯＤ、ＰＯＤ、α－ＡＭＹ进行了研究，分析了其组织特异性，讨论了各同工酶的基因表达谱式，观察到ＥＳＴ同工酶谱复杂，可分成三个区域；ｓ－ＭＤＨ同工酶有二个基因座位，ｍ－ＭＤＨ同工酶不稳定；ＳＯＤ同工酶有三个基因位点，本文还对需进一步研究的问题和各同工酶的应用前景进行了讨论．

False pregnent rats injected with IFN-γ(10U/g) in D2, D4,D6 respectively, changs of plasma progesterone content were determined with RIA.Results show that corpora lutea of different ages have different abillity to secrete progesterone, it secretes considerable amount of progesterone in D5. IFN-γ can increase plasma progesterone content markly. Using organ culture model, addition of exogenous IFN-γ to the culture results in that IFN-γ have luteotrophic and that changes of progesterone secretion have an dose-dependent manner.