不同浓度和纯度川续断皂苷Ⅵ小鼠在体肠吸收比较研究

Study on absorption of asperosaponin Ⅵ of different concentrations and purities in rat′s intestine

目的:比较研究不同浓度和纯度的川续断皂苷Ⅵ在大鼠小肠的吸收情况,初步探讨川续断皂苷Ⅵ的吸收机理。方法:利用大鼠在体肠吸收实验模型,采用HPLC法测定肠循环液中川续断皂苷Ⅵ和酚红的浓度,对川续断皂苷Ⅵ大鼠在体肠吸收特性进行研究。结果:川续断皂苷Ⅵ在大鼠肠道的吸收速率常数(ka)、吸收百分率(pA)随浓度的升高无显著性差异。纯度升高时虽然ka有所降低(P>0.05);但吸收百分率(pA)显著增加(P<0.01)。结论:川续断皂苷Ⅵ提取物中的共存成分可降低其吸收;其吸收方式可能为被动扩散。

Objective ：To observe the absorption of different purities and concentrations of asperosaponin Ⅵ in rat＇s intestine. Methods： HPLC method was used to determine the concentration of asperosaponin Ⅵ in the perfusion which outleted from the opened rat small intestine in rive. Result： No significant increase of rat intestinal absorption was found when of different concentration of asperosaponin Ⅵ were used. Although a slight decrease （ P 〉 0.05 ） on the intestinal absorption rate constant （ ka ） was observed when administration of higher purity of asperosaponin Ⅵ, the absorption rate （pA） increased significantly （P 〈0.01 ）. Conclusion： The components in the extract of Dipsacus asper Wall appears to reduce the intestinal absorption of asperosaponin Ⅵ, whose transportation mechanism may due to passive diffusion.