摘要
目的:用高效液相色谱法测定复方胆通胶囊中羟甲香豆素的含量。方法:高效液相色谱法,采用SWG(200mm×4.6mm,5μm)柱,以萘为内标,甲醇-水(3:1)为流动相,流速为1.00mL·min^-1,检测波长为252nm。结果:羟甲香豆素在17~68μg·mL。浓度范围内呈线性关系(r=0.9999,n=7)。该法回收率99.4%,RSD为0.05%(n=5)。结论:该方法简单、快速、准确。
Objective:Determination of 7-hydroxy 4-methyl coumarin in compound Dantong capsule by HPLC. Method: The applied column was SWG (4.6 mm×200 mm, 5 mm). The internal standard was Naphthalene. The mobile phase consisted of menthol - water (3 : 1 ) at a flow rate of 1.00 mL·min^ -1( The detection wavelength was 252 nm. Results: There was a good linear relationship in the range of 17 -68 μg·mL^-1 (r =0.9999, n =7). The average recovery was 99.4% with RSD of 0.05% (n =5). Conclusion: The method is simple, rapid and accurate.
出处
《药学与临床研究》
2008年第4期333-334,共2页
Pharmaceutical and Clinical Research