SPE-HPLC测定断血流胶囊中醉鱼草皂苷Ⅳb的含量

SPE-HPLC determination of buddlejasaponins Ⅳb in Duanxueliu capsules

目的:建立固相萃取-高效液相色谱法测定断血流胶囊中醉鱼草皂苷Ⅳb的含量。方法:色谱柱:Dikma Kromasil C_(18)柱(5 μm,250 mm×4.6 mm);流动相:甲醇-水(80:20);流速:0.8 mL·min^(-1);检测波长:250 nm;柱温:40℃。结果:醉鱼草皂苷Ⅳb在0.15～1.2μg范围内具有良好的线性关系(r=0.9999),总平均加样回收率为98.8%(n=9)。结论:该方法简便、快速、有效、灵敏、准确,具有良好的重复性和回收率,可作为该制剂的定量分析方法。

Objective：To establish a method for the content determination of buddlejasaponins Ⅳ b in Duanxueliu capsules by SPE -HPLC. Methods：The separation was carried out on a Dikma Kromasil C18 column （5 μm,250 mm × 4. 6 mm）. The mobile phase was methanol - water（ 80： 20）. The flow rate was 0. 8 mL ·min^ -1. The detective wavelength was set at 250 nm. The column temperature was 40 ℃. Results ： The calibration curve showed good linearity over the range of 0. 15 - 1.2 μg（ r = 0. 9999）. The average recovery was 98.8% （ n = 9 ）. Conclusion： This method is simple, rapid, efficient, sensitive, accurate and reproducible, it can be used as a quantitative analysis method for this preparation.