肾细胞癌与良性肾肿瘤组织微血管密度和血管内皮细胞生长因子表达的对比研究

Microvascular density and the expression of vascular endothelial growth factor in renal cell carcinoma and benign tumor tissues

目的:通过检测肾细胞癌、肾脏良性肿瘤标本中的微血管密度(MVD)及血管内皮细胞生长因子(VEGF)表达情况,研究不同性质肾脏肿瘤血管生成的差异。方法:肾细胞癌标本39例,良性肾脏肿瘤标本12例。采用"热点(hotpoint)"法计数MVD,VEGF的定量标准为VEGF染色强度与阳性细胞百分比分值的乘积。结果:MVD、VEGF在肾细胞癌和良性肾脏肿瘤均有表达。肾细胞癌组织MVD(18.3±6.9)计数/视野,VEGF乘积值3.63±2.5(×400),转移者MVD和VEGF乘积值分别为(26.2±7.8)计数/视野、5.83±2.4,未转移者MVD和VEGF乘积值分别为(14.4±4.6)计数/视野、2.06±2.7;12例良性肾脏肿瘤MVD为(2.5±1.3)计数/视野,VEGF乘积值为0.32±0.2。肾细胞癌组织MVD和VEGF表达显著高于良性肾肿瘤(P<0.01),转移组肾细胞癌MVD和VEGF表达显著高于未转移组(P<0.01);肾细胞癌组织MVD与VEGF统计学上呈正相关(r=0.684,P<0.001)。结论:肾细胞癌MVD及VEGF表达均显著高于良性肾脏肿瘤,提示不同性质的肾脏肿瘤其血管生成情况不同,转移肾细胞癌血管生成水平亦高于未转移肾细胞癌,提示血管生成与肾细胞癌转移相关,肾细胞癌组织MVD和VEGF表达水平呈正相关。

Objective To study the difference of the angiogenesis between the renal cell carcinoma （RCC） and benign tumor by detecting the microvascular density （MVD） and the expression of vascular endothelial growth factor （VEGF） in specimens of RCC and benign renal tumors. Methods The specimens of 39 RCC and 12 benign renal tumors were used in the study. The MVD was determined as the mean of microvessel under 5 microscopic vision fields （ × 400）. The VEGF expression was detected by immunohistochemical technique. Results The MVD was about （18.3 ± 6.93）/visual field and the quantified VEGF expression was about 3.63 ± 2.5 in RCC tissue （ × 400）. And the MVD was about （2.5±1.3） / visual field and the quantified VEGF expression was about 0.32 ± 0.2 in benign tumor tissue. The MVD and quantified VEGF expression were ahout （26.2 ± 7.8） / visual field, 5.83 ± 2.4 and （14.4 ± 4.6） / visual field, 2.06 ± 2.7 in metastatic RCC and non-metastatic tissues, separately. The MVD and VEGF expression in RCC were significantly higher than those in benign renal tumors （P 〈 0.01 ）. The MVD and VEGF expressions in metastatic RCC were also significantly higher than those in non-metastatic RCC （P 〈 0.01）. The expressions of MVD and VEGF were statistically correlated in 39 RCC （r = 0.684, P 〈 0.001 ）. Conclusions The expressions of MVD and VEGF in RCC are significantly higher than those in benign renal tumors, which indicats that anglogenesis in RCC is significantly higher than that in benign renal tumors. The expressions of MVD and VEGF in metastatic RCC are also significantly higher than those in non- metastatic RCC, which reveals that angiogenesis may correlate with metastasis. The MVD has a direct correlation with VEGF expression in tissues of RCC.