健骨灵胶囊对维甲酸所致骨质疏松大鼠骨形态与骨代谢的影响

Effect of Jianguling capsule on the bone morphology and metabolism in rats with vitamin A acid-induced osteoporosis

目的:研究健骨灵胶囊对维甲酸致骨质疏松大鼠骨形态和骨代谢的作用。方法:建立维甲酸致大鼠骨质疏松症模型,造模同时灌胃给予健骨灵胶囊0.18,0.36,0.72 g.kg-1.d-1,qd,连续给药29 d后,测定大鼠的骨形态学指标、血液生化学指标及24 h尿中钙(Ca)、磷(P)排泄量。结果:与模型组比较,健骨灵胶囊组大鼠骨小梁平均宽度、骨小梁面积、骨小梁骨细胞数、骨皮质平均宽度、股骨头平均灰度均显著增加;血清Ca、P、碱性磷酸酶(ALP)、骨钙素(BGP)、雌二醇(E2)、睾酮(T)的含量显著增加,甲状旁腺激素(PTH)水平显著降低;尿中Ca和P排出量显著减少,大鼠股骨、卵巢和睾丸系数显著增加,大鼠的性腺组织学结构不同程度的得到改善。健骨灵胶囊的作用呈现一定的剂量依赖性。结论:健骨灵胶囊对维甲酸致骨质疏松大鼠的骨形态和骨代谢有一定的改善作用。

Objective： To investigate the effect of Jianguling capsule（JC） on the bone morphology and metabolism in rats with vitamin A acid-induced osteoporosis.Methods： Osteoporosis was induced by vitamin A acid in rats,and JC（0.18,0.36 and 0.72 g·kg^-1·d^-1） was orally administered to the osteoporotic rats for 29 days.The bone morphological parameters,blood biochemical indexes and 24-h excretion of calcium and phosphorus in urine were detected.Results： Compared with the control osteoporotic rats,JC markedly increased following variables： the average width of bone trabecula,bone cell number of trabecula,area of bone trabecula,average thickness of bone cortex,and average gray degree of femoral caput,the serum levels of calcium,phosphorus,alkaline phosphatase（ALP）,osteocalcin（BGP）,estrogen and testosterone,the coefficients of thighbone,ovary and testicle.Meanwhile,JC decreased the serum level of parathyroid hormone（PTH）,and the excretion of urine calcium and phosphorus in urine.Also,JC improved the histological structures of sex glands.JC effect was related to the doses used.Conclusion： JC can ameliorate the bone morphology and metabolism in rats with vitamin A acid-induced osteoporosis.