热毒宁注射液对急性肺损伤家兔肺表面活性物质的影响

Effect of Reduning on lung surfactant in rabbits with acute lung injury

目的:研究热毒宁注射液(RDN)对急性肺损伤(ALI)家兔肺表面活性物质磷脂酰胆碱含量(PC)的影响。方法:健康家兔分3组,静脉注射内毒素(主要成分为脂多糖,LPS)建立ALI模型,应用RDN治疗后,不同时间点检测外周血及支气管肺泡灌洗液(BALF)内白介素-8,10(IL-8,IL-10),肿瘤坏死因子(TNF-α)的含量,并测定BALF内PC的含量。结果:LPS诱导的ALI家兔中,血和BALF中的致炎因子含量较正常对照组显著升高,肺通透指数升高,PC较正常对照组显著下降[(6.43±2.44)vs(4.24±0.93)mg.g-1,P<0.01]。与模型组比较,RDN治疗组可显著逆转上述各指标的异常变化,PC显著升高[(4.24±0.93)vs(5.91±2.04)mg.g-1,P<0.05]。结论:RDN对家兔ALI有一定的治疗作用,作用机制不仅与抑制致炎因子的活性有关,还可能与增加ALI家兔肺表面活性物质PC的含量有关。

Objective： To investigate the effect of Reduning （RDN） on lung surfactant in rabbits with acute lung injury （ALI）. Methods： Healthy rabbits （n = 30） were randomly divided into 3 groups （n =10 for each group）, and endotoxin （ mainly consisted of LPS） was iv injected to induce ALI. Blood was harvested before （0 h） and 1 h after two LPS injections （25 and 49 h）. Bronchoalveolar lavage fluid （BALF） was collected at 49 h. Lung permeability index, IL-8, IL-10, TNF-α, and BALF surfactant （phosphatidyl choline, PC） were detected. Results： LPS induced ALI in rabbits. IL-8, IL-10, TNF-α were obviously higher, and PC in BALF was lower [ （6.43±2.44） vs （4.24±0.93） mg·g^-1, p 〈 0.01 ], in ALI rabbits than in normal controls. RDN obviously attenuated the changes in these indicators [PC： （4.24±0.93） vs （5.91±2.04） mg·g^-1, P〈0.05]. Conclusion： RDN has protective effects in rabbit with ALI; its mechanism may relate to the inhibition of cytokines and increases of PC in surfactants.