溃疡性结肠炎患者结肠黏膜HBD-2和NF-κB及IL-8的表达

Study on the expression of HBD-2,NF-κB,IL-8 and the significance in colonic mucosa of ulcerative colitis

目的研究β-防御素2(HBD-2)、核因子-κB(NF-κB)、白细胞介素-8(IL-8)在溃疡性结肠炎(UC)患者结肠黏膜中的表达,探讨HBD-2、NF-κB和IL-8在UC发病中的作用和相互关系。方法收集30例UC患者和10例正常结肠黏膜组织,用免疫组织化学法检测HBD-2、NF-κB和IL-8蛋白表达。结果免疫组织化学分析显示,UC组HBD-2、NF-κB和IL-8表达阳性率和表达强度明显高于正常结肠黏膜组(P<0.01);UC轻中重度三组结肠黏膜中HBD-2、NF-κB和IL-8表达阳性率和表达强度比较差异无统计学意义(P>0.05)。结论HBD-2、NF-κB和IL-8水平在UC结肠黏膜组织中明显升高,但与UC病情轻重关系不密切。

Objective To detect the expression of human β-defensin 2 （HBD-2） and nuclear factor kappa B（NF-κB） and Interleukin-8 （IL-8） in colonic mucosa of ulcerative colitis （UC） patients and to evaluate the role of HBD-2,NF-κB, IL-8 and their relationship were studied. Methods The colonic mucosa from 30 UC patients and 10 normal persons were investigated. The expression of HBD-2, NF-κB and IL-8 was measured by immunonohistochemistry. Results In immunohistochemical staining, the positive rate and the intensity of the expression of HBD-2 , NF-κB and IL-8 in the colonic mucosa of UC was significantly higher than that in the normal colonic mucosa（P〈0.01）. The positive rate and the intensity of the expression of HBD-2 ,NF-κB and IL-8 had no statistical difference in colonic mucosa of mild,moderate and serious UC（P〉0.05）. Conclusions The protein expressions of HBIG2,NF-κB and IL-8 were significantly increased in UC, but it had no correlation with the severity of clinical symptom of patients With UC.