蝎毒耐热蛋白对外源性Aβ_(1-40)神经突触毒性的抑制作用

The Inhibition of Scorpion Venom Heat-resistant Protein on Synapse Toxicity of Neurons by Ectogenic Aβ_(1-40)

目的探讨蝎毒耐热蛋白(SVHRP)对Aβ1-40神经突触毒性的影响。方法应用Aβ1-40进行大鼠海马内定位注射后,腹腔给予SVHRP进行干预。10d后,采用水迷宫实验和免疫组织化学方法分别检测大鼠的学习记忆能力和海马内突触密度的变化。结果SVHRP干预组大鼠的水迷宫逃避潜伏期缩短、目标象限游泳时间和距离增加;SVHRP干预组大鼠的P38免疫反应产物明显强于Aβ组,接近对照组水平。结论腹腔注射SVHRP可抑制外源性Aβ1-40引起的大鼠海马内突触密度的下降,可阻止Aβ1-40对大鼠学习记忆能力的损害。

Objective To investigate the intervention of scorpion venom heat-resistant protein（SVHRP） on synapse toxicity of neurons by A β1-40 Methods The A β 1-40 was injected into hippocampus of rats and then SVHRP was applied intraperitoneally, Ten days later, Morris water maze was used to measure the spatial learning and memory ability of rats. The expression of synaptophysin （P38） was detected by immunohistochemistry. Results The escape latency of SVHRP group became shorter, and the swimming distance and time percentage of SVHRP group in the platform quadrant were longer ,compared with A β group. The P38-positive expression in hippocampus was weaker and thiner in A β injection group than in normal control group（P〈 0.05, however, it was similiar in SVHRP group to control group. Conclusions SVHRP can markedly inhibit the decrease of synapse density caused by A β1-40 injection in hippoeampus, and improve learning and memory impairment of rats with A β injection.