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人乳腺癌中树突状细胞的形态学变化及其与DNA含量的关系

Morphological Changes of Dendritic Cells and their Relation with DNA in Human Breast Cancer
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摘要 目的利用CD1C作为树突状细胞的特异性标志分子,研究乳腺癌患者树突状细胞的形态学变化及其与DNA含量的关系,探讨CD1c+DC与乳腺癌生物学特性的关系,为乳腺癌的生物治疗提供实验依据。方法收集手术切除的人乳腺癌组织和癌旁相对正常乳腺癌组织,用免疫组织化学方法和流式细胞术对20例乳腺癌标本进行检测。结果乳腺癌组织中CD1C的表达强度较相对正常乳腺组织明显减弱(P<0.05),CD1C+DC的数量较相对正常乳腺组织明显减少(P<0.05),CD1C+DC的数量和乳腺癌细胞内DNA指数(DI)呈负相相关,CD1C+DC的数量增多时,则DI值减小。结论树突状细胞是专职的抗原呈递细胞,在抗乳腺癌的免疫反应中起着重要作用。 Objective The purpose of this article is to study the morphological changes and the correlation between the number of CD1C＋DC and breast cancer biological behavior to provide the experimental data for the further biological therapy of breast cancer, Methods The human breast cancer tissues and relatively normal breast tissues were collected from surgical operation and the samples of 20 patients with breast cancer were analyzed by immunocytochemistry and flow cytometry（FCM） using CD1C monoclonal antibody that specifically recognizes CD1C molecule expressed by DC cells lineage. Results The expressive intensity of CD1C was dramatically weaker（P〈0.05）, the number of CD1C＋DC reduced greatly in breast cancer tissue than in relatively normal breast tissues（P〈0.05）. There is a negative feedback loop between number of CD1C＋DC and DNA content, DNA index decreased while the number of CD1C＋DC increased. Conclusion Dendritic cells may play an important tole in initiating immune responses against breast cancer.
作者 李艳萍 王琳 史福军 许梅
机构地区 新乡医学院组织学与胚胎学教研室 上海交通大学神经生物实验室
出处 《解剖科学进展》 CAS 2008年第3期267-269,273,共4页 Progress of Anatomical Sciences
基金 河南省科技厅科技攻关项目(991170208)
关键词 树突状细胞 CD1C 流式细胞术 乳腺癌 dendritic cells： CD1C flow cytometry breast cancer
分类号 R737.9 [医药卫生—肿瘤]
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参考文献11

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