阻断交感神经对小鼠小肠黏膜免疫相关细胞的影响

The Role of Sympathetic Nerve on The Immuno-associated Cells in Small Intestinal Mucosa of Mice

目的研究交感神经对肠道黏膜屏障的调节作用。方法采用常规组织学和免疫组织化学技术,观察经腹腔注射交感神经损毁剂6-羟多巴胺后小鼠小肠上皮内淋巴细胞、杯状细胞、IgA+浆细胞、肥大细胞的数量分布变化。结果注射6-羟多巴胺组小鼠与对照组小鼠相比,小肠各肠段的上皮内淋巴细胞减少了5.6%～33.8%,杯状细胞减少了10.6%～18.5%,两组指标在十二指肠之间和回肠之间差异极显著(P<0.01);肥大细胞增加了15.0%～57.1%,在空肠之间差异极显著(P<0.01);IgA+浆细胞增加了13.9%～61.4%,在十二指肠之间差异显著(P<0.05)。结论交感神经对调节小鼠肠道的免疫学屏障有着重要作用。

Objective To explore the regulatory effect of sympathetic nerve on the mucosal barrier of small intestine of mice. Methods The model of sympathectomy mouse was established by injection of 6-OHDA intrapefitoneally. The intraepithelial lymphocytes, the goblet cells, IgA^＋ cells and mast cells in intestinal villi were observed using histology and immunohistoehemistry methods. Results In the sympathectomy groups compared with the control group, the number of the intraepithelial lymphoeytes decreased by 5.6% - 33.8% （P〈0.01）, the number of the goblet cells decreased by 10.6% 18.5%P〈0.01） in duodenum and ileum with obvious difference between duodenum and ileum, the number of the mast cells increased by 15.0% - 57.1%P〈0.01） in jejunum, the number of the IgA^＋ cells increased by 13.9% - 61.4% （P〈0.05） in duodenum. Conclusion The sympathetic nerve has important role in the modulation of the mucosal immune barriers in the intestine of mice.