摘要
目的研究少突胶质细胞(oligodendrocytes,OL)与2型星形胶质细胞(type-2astrocyte,T2A)中S-100的表达。方法根据各种胶质细胞的生长时间的差异及细胞的黏附性不同,通过机械振荡法和差速贴壁法获得纯化的O-2A祖细胞;免疫细胞化学染色法观察S-100β在O-2A谱系细胞中的表达情况。结果O-2A祖细胞在含有PDGF、bFGF的培养液中培养3d,分化成T2A,培养5d分化成OL;OL中S-100β免疫反应呈现阳性,T2A中不表达S-100β。结论O-2A谱系细胞中的S-100β的表达与O-2A祖细胞向OL分化有关。
Objective To investigate the expression of S-100βin the oligodendrocytes（OL） and type-2 astrocyte（T2A）. Methods Based on the differential properties of cellular adhesions and developmental time-course, O-2A progenitor cells were obtained by mechanical shaking and differential adhesion method. S-100β expression was detected by immunocytochemistry staining. Results O-2A progenitor cells were cultured and differentiated into oligodendrocytes （5d culture） and type-2 astroeytes （3d culture） in the presence of PDGF and bFGF. However, S-100 β expression was found in oligodendrocytes, but not seen in type-2 astrocytes. Conclusion The expression of S-100 β in O-2A lineage cells may be correlatd with the differentiation of the O-2A progenitor cells into the OL.
出处
《解剖科学进展》
CAS
2008年第3期309-311,共3页
Progress of Anatomical Sciences
基金
江苏省高校自然科学研究计划(05KJB310121)
