反硝化聚磷菌的SBR反应器中微生物种群与浓度变化

Change of microorganism amount and species in SBR reactor enriching denitrifying phosphate bacteria

依据DPB原理,以SBR反应器富集反硝化聚磷菌,进行各阶段的泥水混合液中微生物浓度与种群变化的研究。研究结果表明:聚磷菌\反硝化聚磷菌的浓度分别增加为原来的94和75倍。第2段运行后,常规聚磷菌和部分放线菌被淘汰,污泥沉降比(SV)的变化为反应器细菌变化提供了指示作用。好氧段中硝化菌、亚硝化菌浓度明显比厌氧段的高。好氧段中亚硝化菌下降为原来的0.63倍,但硝化菌的浓度增加为最初的19.3倍。反硝化菌、聚磷菌的浓度比反硝化聚磷菌多。反硝化菌的浓度先上升后下降,第2段淘汰了常规的反硝化菌。反应器中有一定量的发酵菌和产乙酸菌,但无产甲烷菌。富集后聚磷菌的种类减少且集中,反硝化聚磷菌以假单胞菌属、棒状杆菌属为主,肠杆菌科和葡萄球菌属次之。

Based on the DPB（denitrifying phosphorus removal bacteria） principal, the SBR（sequencing batch reactor） reactor was used to enrich DPB, The change of microorganism amount and species during different phases were studied, The result shows that the amounts of PAO（phosphate accumulation organism） and DPB increase 94 and 75 times, respectively. After the second phase, the routine PAO and partial actinomyces are eliminated, The change of SV（sludge setting ratio） provided the indication of the bacteria change. The amount of nitrate and nitrite bacteria during the aerobic phase is larger than that of anaerobic phase. The amount of nitrite bacteria decreases 0.63 times but the nitrate bacteria increases 19.3 times. The amount of denitrifying bacteria and PAO is more than that of DPB. The amount of denitrifying bacteria rises first but then drops. The routine denitrifying bacteria are eliminated during the second phase. The reactor has some zymogen and acetogen, but no methanogen bacteria. After enrichment the species of PAO are reduced and concentrated. DPB includes Pseudomonas spp/Corynebacterium spp/Enterobacteriaceae spp and Staphylococcus spp.