喉癌中血管内皮生长因子C和微淋巴管密度的关系

Relationship of VEGF-C expression and lymphatic microvessel density with cervical lymph node metastasis in human laryngeal cancer

目的分析血管内皮生长因子C(vascular endothelial growth factor C,VEGF-C)、微淋巴管密度与颈淋巴结转移的关系,以及VEGF-C的表达与微淋巴管密度的相关性。方法采用免疫组化SP法,检测47例喉癌组织及15例声带息肉中的VEGF-C的表达;检测47例喉癌组织、癌旁组织中的微淋巴管密度。结果VEGF-C在Ⅰ、Ⅱ、Ⅲ级病理分级的阳性率分别为55%、72.22%、88.89%,表达与肿瘤分化明显相关(P<0.05)。VEGF-C在淋巴结转移组和非转移组的阳性率分别为82.35%和60%,差异显著(P<0.05)。喉癌组织微淋巴管密度在淋巴结转移组与非转移组分别为15.38±5.68和10.41±4.23,差异非常显著(P<0.01)。结论VEGF-C的表达与喉癌组织微淋巴管密度及喉癌的淋巴结转移密切相关。

OBJECTIVE To analyze the relationship of VEGF-C expression, lymphatic microvessel density and cervical lymph node metastasis in human laryngeal cancer, as well as the relationship of VEGF-C expression and lymphatic microvessel density. METHODS VEGF-C expression and lymphatic microvessel density were detected in 47 laryngeal cancer tissues with SP immunohistochemical method. RESULTS The positive rates of VEGF-C in the Ⅰ、Ⅱ、Ⅲ pathological grade level were 55 %, 72.22 %, 88.89% respectively, and its expression was significantly correlated with tumor differentiation（P〈 0.05）. The positive rates of VEGF-C in lymphatic metastasis group and non-metastasis group were 82.35 % and 60 % respectively, and it had significant difference （P 〈 0.05）. Lymphatic microvessel density of the Laryngeal cancer tissue in lymph node metastasis group and nonmetastasis group were 15.38±5.68 and 10.41 ±4.23 respectively, and it had significant difference （P 〈 0.01）. CONCLUSION VEGF-C expression and the lymphatic microvessel density of the laryngeal cancer tissues are closely related to cervical lymph node metastasis.