16S rRNA/DNA-PCR对初生新生儿肠道乳酸杆菌和大肠埃希菌的动态检测

Study on the variation of Lactobacillus and Escherichia coli in earlier newborn babies by fluorescent quantitative 16S rRNA/DNA targeted PCR

目的观察新生儿生后肠道菌群的动态变化。方法采用16S rRNA/DNA荧光定量PCR技术,分别对40例足月儿和40例早产儿生后第1(>12h)、4、7天粪便标本中的乳酸杆菌和大肠埃希菌进行定量分析。结果不同日龄足月儿粪便标本中乳酸杆菌数量的对数值分别为5.50±0.81、6.87±0.81、9.20±0.87,早产儿分别为4.89±0.46、6.05±0.46、8.06±0.18;不同日龄足月儿粪便标本中大肠埃希菌数量的对数值分别为6.49±0.40、7.59±0.58、7.15±0.55,早产儿分别为6.32±0.51、7.39±0.78、7.05±0.63;2种细菌对数值分别在组间行单因素方差分析,差异有显著性(P<0.05);组内行配对t检验,差异也有显著性(P<0.05)。结论新生儿肠道菌群的建立是动态变化的,个体间差异受多因素影响,早产儿落后于足月儿。

Objective To observe the variation of intestinal flora in newborn babies. Methods The amount of stool Lactobacillus and Escherichia coli of first day, fourth day, seventh day after birth in 40 term and 40 preterm babies were measured by the practical fluorescent quantitative 16S rRNA/DNA-targeted PCR. Results Lactobacillus logarithmic abso- lute value of first day, fourth day, seventh day after birth were 5.50 ± 0.81,6.87 ± 0.81,9.20 ± 0.87 in term babies re- spectively, and 4.89 ± 0.46,6.05 ± 0.46,8.06 ±0.18 in preterm babies respectively. Escherichia coli logarithmic absolute value of first day, fourth day, seventh day after birth were 6.49 ± 0.40,7.59 ± 0.58,7.15 ± 0.55 in term babies respective- ly, and 6.32 ±0. 51 ,7.39± 0. 78,7.05 ± O. 63 in preterm babies respectively. The above values had statistical difference between group-to-group and within group （ P 〈 0.05 ）. Conclusions There are developmental variation of intestinal flora formation in newborn babies. The intestinal bacterial flora formation of the preterm babies is slower than that of tenn.