有机磷诱导HUVEC-12损伤的实验研究

The damage on human umbilical vein endothelial cell lines induced by trichlorfon

目的观察有机磷农药敌百虫对人脐静脉内皮细胞(HUVEC-12)损伤的时相特征,探讨敌百虫诱导内皮细胞损伤的作用机制。方法将HUVEC-12与含不同浓度敌百虫的DMEM培养基孵24h后,MTT法检测细胞的活性;以IC50浓度的敌百虫处理细胞一定时间后(0、4、6、8、12、24、48h),观察敌百虫诱导内皮细胞损伤的时相特征;硫代巴比妥法检测细胞上清液中丙二醛(MDA)浓度。结果敌百虫呈时间依赖性地促进内皮细胞凋亡及细胞上清液MDA产生。结论敌百虫刺激HUVEC-12氧化应激,致细胞损伤。

Objective To explore the time-effect of damage in human umbilical vein endothelial cell lines induced by Trichlorfon and to analyze its mechanism. Methods HUVECs-12 were cultured in DMEM containing Trichlorfon for 24 h, the growth inhibition was measured by MTT assay. Cells were incubated in IC50 concentration Trichlorfon （400 μmol/L） for different times（0,4,6,8,12,24,48 h） ） to test the time-effect of damage in human umbilical vein endothelial cells ; the level of malondialde-hyde （MDA） in supernatant-condi-tioned media was monitored by thiobituric acid reaction （TBA）. Results Trichlorfon inhibited HUVECs-12 growth in a dose-dependent manner and increased the production of MDA. Conclusion Trichlorfon can induce damage in human umbilical vein endothelial cells.