黄芪对大鼠应激性胃溃疡的预防作用

Protect Effect of Radix Injection Fluid on Stress Ulcer in Rats

目的观察黄芪注射液对应激状态下大鼠胃黏膜损伤有无保护作用。方法采用水浸束缚应激方法复制大鼠应激性溃疡模型。将40只健康大鼠随机分为5组:对照组、模型组、黄芪10g/kg、20 g/kg 组和硫糖铝500 mg/kg 给药组。分别连续灌胃给予黄芪或硫糖铝5 d 后复制模型,6 h 后取胃计算溃疡指数,采用光镜观察大鼠胃组织病理改变。结果水浸束缚应激致应激性溃疡形成,胃黏膜出现广泛的糜烂、出血。黄芪和硫糖铝均可显著降低溃疡指数(P<0.01),其中黄芪20g/kg 疗效最佳,优于硫糖铝500 mg/kg(P<0.05)。黄芪可显著减轻大鼠胃黏膜细胞损伤程度和毛细血管及其周围间质的水肿。结论黄芪对应激性溃疡大鼠具有显著的胃黏膜保护作用。

Objective To investigate the protective effect of radix injection fluid on stress ulcer in rats. Methods Water immersion and restraint stress （WRS） induced acute gastric injury was used as the experiment model of stress ulcer of rats. All the animals were randomly divided into 5 groups,the control group,the model group,radix injection fluid 10 - 20 g/kg and sucralfate 500 mg/kg group. Rats were orally administrated with radix injection fluid or sucralfate for 5 days before stressed. 6 hours after WRS, rats were anesthetized and the stomachs were removed to examined the ulcer index as the method described. Morphological observation of the change of pathology in gastric mucosa was performed by light microscopy. Results WRS 6 hours resulted in acute gastric mucosal lesion characterized by extensive erosion,hemorrhage and ulceration. The administration of radix or sucralfate could significantly decrease the ulcer index （ P 〈 0. 01 ）. Radix 20 g/kg exerted the best protective effect and superior to sucralfate 10 g/kg （ P 〈0. 05）. Radix could lessen the mucosal damage and alleviate the edema in capillary vessel and interstitial tissue. Condusion Radix exhibited remarkable protective effect on WRS induced stress ulcer in rats.