血管紧张素转换酶2基因A9570G多态性与2型糖尿病合并冠心病的相关性研究

Association between the Polymorphism of A9570G in Angiotensin I Converting Enzyme 2 Gene and Type 2 Diabetes Complicated with Coronary Heart Disease

目的:研究血管紧张素转化酶2(angiotensin I converting enzyme2,ACE2)基因A9570G多态性与2型糖尿病(type2diabetes,T2DM)合并冠心病(coronary artery disease,CHD)的相关性。方法:选择广东地区无血缘关系的T2DM患者共262例,所有患者按是否并发CHD分为T2DM+CHD组和T2DM组,对照组100例为健康体检者,应用聚合酶链反应和限制性内切酶片段长度多态性的方法检测ACE2基因A9570G多态性,综合分析ACE2基因A9570G多态性与T2DM并发CHD的关系。结果:在男性,对照组和T2DM组等位基因频率及基因型构成差异均无统计学意义(χ2=0.024,P=0.876),但T2DM+CHD组G等位基因频率及G基因型频率高于对照组及T2DM组,差异有统计学意义(χ2=4.781,P=0.029及χ2=4.933,P=0.026);在女性,3组间等位基因频率及基因型构成差异均无统计学意义(χ2=0.037,P=0.981及χ2=1.243,P=0.871)。根据不同基因型对T2DM+CHD组患者进行亚组分析发现,在男性,G基因型患者冠脉病变的Genisini积分高于A基因型者,差异有统计学意义(t=2.243,P=0.029);在女性,则未观察到这种差异(F=1.156,P=0.324)。结论:ACE2基因A9750G多态性可能与男性T2DM并发CHD的遗传易感性相关,并且与其冠脉病变程度有一定关系。

Objective：To explore the association between the polymorphism of A9570G in angiotensin I converting enzyme 2 gene and type Ⅱ Diabetes （T2DM） complicated with coronary heart disease（CHD）.Methods：Two hundred and sixty two pa tients with T2DM were included in the study. All the patients were divided into T2DM group and T2DM ＋ CHD group accord ing to with or without CHD, and control group included one hundred healthy persons. Following the principle of case control , polymerase chain reaction and restriction fragment length polymorphism（PCR RFLP） were used to observc the association of polymorphism of A9570G in ACE2 gene with T2DM complicated with CHD. Results： The genotyping data indicated that the allele frequencies and the construction of genetype frequencies in male subjects had no difference between control group and T2DM group （X^2= 0. 024,P=0. 876）, but the G allele frequencies and G genotypes frequencies in T2DM ＋ CHD group was significantly higher than control group and T2DM group（ X^2=4.781 , P =0.029 and X^2= 4.933, P=0.026） ; the allele frequencies and the construction of genetype in female subjects had no difference among three groups （X^2=0.037,P=0.981 and X^2=1.243.P=0.871）. Subset analysis based on different genetypesin T2DM＋CHDgroupindicated that Genisini score in patients with G genetype is significantly higher than those in patients with A genetype in male（t=2.243,P=0.029） ,but this difference was not observed in female（F = 1.156.P = 0.324）. Conclusion：The polymorphism of A9570G in ACE2 gene may be associated with the genetic predisposition of Type 2 diabetes complicated with coronary heart disease in male ,and it is also associated with the the severity of coronary atherosclerosis.