摘要
目的:探讨血管生成素及受体酪氨酸激酶(Angs及Tie-2)与髓系白血病的关系,以及与血管内皮生长因子(VEGF)的相互关系。方法:应用半定量RT-PCR检测80例急性髓系白血病(AML)、10例慢性髓系白血病(CML)和10例正常人骨髓单个核细胞(BMNCs)中Ang-1、Ang-2、Tie-2和VEGF mRNA的表达。用流式细胞术检测白血病细胞增殖指数(PI)。结果:与正常对照相比,除Ang-1外,Ang-2、Tie-2和VEGF mRNA在初治的髓系白血病中表达均增高,缓解后降低。在Ang-2与VEGF双阳性白血病组,髓外浸润率明显增高(P<0.05);Ang-2/Tie-2双阳性组缓解率低(P<0.05)、PI增高(P<0.05)并与CML的发展阶段有关。结论:髓系白血病患者高表达Angs/Tie-2,同时其与VEGF之间相互作用,与白血病的发展阶段相关。
Objective:To investigate the expression level of Angs/Tie-2 and VEGF mRNA and their significance in myeloid leukemia. Methods:The expression of Ang-1, Ang-2, Tie-2 and VEGF mRNA in 80 AML patients, 10 CML patients and 10 healthy controls were detected by RT PCR. The Proliferation Index (PI) of leukemia ceils was assayed by flow cytometry (FCM). Results: The expression levels of Ang-2, Tie-2 and VEGF mRNA, except Ang-1 mRNA, were statistically significant higher in untreated myeloid leukemia patients than those in complete remission (CR) patients. The percentage of extra-medullary infiltrations were significant higher in both Ang-2 and VEGF positive group (P〈0.05). Patients with both Ang-2 and Tie- 2 positive had a lower CR rate (P〈0.05) but a higher PI (P〈0.05). The expression levels of Ang-2 and Tie-2 increased significantly with the progressing of CML. Conclusion: Angs/Tie-2 mRNA are over-expressed in myeloid leukemia. The over-expressed Angs/Tie-2 may have synergistic effect with VEGF in the pathogenesis of leukemia.
出处
《中国临床医学》
北大核心
2008年第4期518-521,共4页
Chinese Journal of Clinical Medicine