微流控芯片测定滴鼻液中的盐酸萘甲唑啉

Determination of naphazoline hydrochloride in naphazoline hydrochloride nose drops by microfluidic chip

建立了微流控芯片非接触电导检测法测定盐酸萘甲唑啉的分析方法。探讨了缓冲液种类、浓度，添加剂种类、浓度以及分离电压等因素对分离检测的影响。优化选择20mmol／L H3BO3＋10mmol／L Tris（pH7．5）缓；中溶液，2mmol／L β-CD添加剂．分离电压2．70kV时，3min内可实现盐酸萘甲唑啉的快速分离检测。在优化条件下，盐酸萘甲唑啉的线性范围为20～1．0×10^3μg／mL（r=0．995），检出限为5．0μg／mL（S／N=3），RSD为2．5％，加标回收率为98．3％～101％。

A method for the determination of naphazoline hydrochloride in naphazoline hydrochloride Nose Drops by mierofluidie chip with eontaetless conductivity detection was established in this paper. The eleetrophoretie parameters, such as the variety and concentration of buffer solution and additive, separation voltage ete, were researched. Naphazoline hydrochloride was separated and detected in the buffer solution of 20 mmol/L H3BO3 ＋ 10 mmol/L Tris （pH 7.5） with 2 mmol/L β-CD at a separation voltage of 2.7 kV within 3 min. The linear dependence of the concentration of naphazoline hydroehloride ranged from 20 μg/mL to 1.0 × 10^3 μg/mL （ r = 0. 995）, the limit of detection （LOD） reached 5.0 μg/mL （S/N= 3）, the RSD was 2.5% and the recoveries were 98.3% - 101%. The method was effective, simple and sensitive, and it could be used in the quality control of these products.