初始接种量对四脊裸胞壳Dh菌株菌丝生物量的影响

Effect of amount of initial inoculation on mycelia biomass of Emericella quadriclineata (Thom & Raper) Bejami Dh

在温度（27±1）℃和光照12L：12D的条件下，用PD培养液对四脊裸胞壳（Emericella quadriclineata（Thom＆Raper）Bejami）Dh菌株进行液体振荡培养（100r／min），测定不同接种量和液体培养时间对菌丝生物量的影响．结果表明：将含干菌丝生物量为23．8mg／mL的母液按1％～15％（V／V）的比例接种后，培养60h以上，1％～15％各接种量处理均能产生21mg／mL以上的菌丝生物量．通过逻辑斯蒂生长模型拟合，发现该菌在上述液培条件下的最大菌丝生物量为36．7mg／mL左右．

Effect of amount of initial inoculation on mycelia biomass of Emericella quadriclineata （Thorn ＆ Raper） Bejami Dh was investigated with shaking （100 r/min） culture of potato dextrose fluid at （27±1） ℃ and 12L ： 12D. Seed culture containing 23.8 mg dry mycelia per milliliter was used to initiate 50 mL liquid culture at the proportions （V/V） of 1%-15 %. After 60 h of incubation the cultures initiated 1% to 15 % seed culture yielded a mycelial biomass higher than 21 mg/mL. The fitting of the data to Logistic growth model found that Emericella quadriclineata （Thom ＆ Raper） Bejami Dh could produce a maximum biomass of 36. 7 mg/mL at the above regime.