原发性高血压患者外周血单核细胞分泌肿瘤坏死因子α与白细胞介素6水平及洛伐他汀干预实验

Incread Secrection of Tumour Necrosis Factor Alpha and Interleukin 6 Induced by Lovastatin in Peripheral Blood Monocytes of Patients with Essential Hypertension

目的观察原发性高血压患者体外培养的外周血单核细胞(PBMC)分泌肿瘤坏死因子α(TNF-α)、白细胞介素6(IL-6)水平及洛伐他汀干预TNF-α、IL-6水平的效果。方法选取原发性高血压患者31例,健康自愿者22例作为对照组。体外培养外周血单核细胞后,①脂多糖刺激PBMC分泌TNF-α、IL-6;②洛伐他汀干预PBMC。酶联免疫法测定细胞培养上清液中的TNF-α、IL-6水平。结果①原发性高血压患者PBMC分泌TNF-α、IL-6水平高于血压正常者PBMC分泌TNF-α、IL-6水平(P<0.05);②脂多糖刺激后两组TNF-α、IL-6均较自发分泌时明显升高(P<0.05)。与正常对照组相比,原发性高血压患者脂多糖刺激PBMC分泌TNF-α、IL-6增加(P<0.05);③洛伐他汀干预后,两组均明显较脂多糖刺激和自发分泌时的TNF-α、IL-6少(P<0.05)。结论原发性高血压患者PBMC处于炎症激活状态,炎症可能参与原发性高血压的发生发展;洛伐他汀可以减轻其炎症反应。

Objective The study was to detect the level of tumour necrosis factor alpha（TNF-α） and interleukin 6 （ IL- 6） in patients with essential hypertension（EH） before and after treatment with lovastatin. Methods 31 patients with EH were enrolled into lovastatin group, and 22 healthy volunteers into control group. PBMC （peripheral blood monocytes） were cultured in vitro and stimulated by lipopolysaccharide （LPS） and lovastatin. The concentrations of TNF-α and IL-6 were compared in PBMC culture supernatant. TNF-α and IL-6 were measured by enzyme -linked immunosorbent assay（ELISA）. Results ① The level of TNF-α and IL-6 of PBMC was significantly increased in patients with EH compared with that in the control subjects （P 〈0.05） . ②After stimulated by LPS, TNF-α and IL-6 had been upregulated in both groups （ P 〈 0.05 ）, moreover TNF-α and IL-6 of the EH group were higher than that of control group （ P 〈 0.05 ）. ③ TNF-α and IL-6 were dramatically inhibited by lovastatin in both groups （ P 〈 0. 05 ）. Condusion Activated PBMC with increased TNF-α and IL-6 secrection may be involved in the pathogenesis of essential hypertensive. It is possible that lovastatin therapy EH via this inflammatory pathway.