nm23H_1蛋白的表达与肺鳞癌转移关系的研究

Relationship between nm23H_1 expression and lymph node metastasis in squamous cell carcinoma of the lung

目的检测肺鳞癌中nm23H1蛋白的表达情况,以探讨nm23H1的表达与肺鳞癌淋巴转移之间的关系。方法收集50例肺鳞癌组织及癌旁正常肺组织,采用免疫组化染色方法检测nm23H1蛋白的表达情况。结果50例肺鳞癌组织中,nm23H1蛋白阳性表达29例,nm23H1蛋白阳性表达率在TNMⅠ期(73.91%)、无淋巴结转移者(72.00%)和单个淋巴结转移者(69.25%)显著高于TNMⅢ期(33.33%)、淋巴结转移者(52.00%)和多个淋巴结转移者(16.67%)(均P<0.05)。另外,nm23H1蛋白阳性表达率与患者年龄,肿瘤部位、大小、分化程度、类型及患者吸烟指数均无关。结论肺鳞癌患者nm23H1蛋白的表达与肺鳞癌淋巴结转移关系密切,nm23H1基因可能在抑制肺鳞癌的淋巴转移方面具有重要作用。

Objective To evaluate the relationship between nm23H1 expression and lymph node metastasis in squamous cell carcinoma of the lung. Methods Specimens of cancer tissue and paracancerous tissue were collected from 50 cases of squamous cell lung carcinoma. The expression of nm23H1 was assessed by immunohistochemistry technique. Results Positive expression of nm23H1 was detected in 29 out of 50 cancer samples. The nm23H1 expression was higher in cases of stage Ⅰ, TMN NO and with single lymph node metastasis than that in cases of stage Ⅲ, TMN N1＋N2 and with multiple lymph node metastasis, respectively （P 〈 0.05）. However, the expression of nm23H1 was not correlated with location, size, differentiation and macroscopic type of tumor; not correlated with age and smoking status of patients. Conclusion Expression of nm23H1 is closely related to lymph node metastasis, which indicates its role in the inhibition of lymph node metastasis.