急性冠脉综合征患者外周血单个核细胞CD40、CD40L的表达

Expression of CD40 and CD40L on peripheral blood mononuclear cells in patients with acute coronary syndromes

目的探讨外周血单个核细胞表达免疫介质细胞分化抗原40(CD40)及其配体CD40L与急性冠脉综合征(ACS)的关系。方法采用间接免疫荧光流式细胞术和酶联免疫吸附法(ELISA)测定ACS组(23例)、稳定性心绞痛(SA)组(16例)和对照组(15例)3组外周血单个核细胞表达CD40、CD40L和血清C-反应蛋白(CRP)水平。结果ACS组外周血单个核细胞表达CD40平均荧光强度(MFI)19.12±4.96明显较SA组9.99±2.55和对照组11.50±2.21高,3组相比差异有统计学意义(F=34.137,P<0.05)。ACS组CD40LMFI0.21±0.27与SA组0.10±0.18、对照组0.19±0.25相比差异无统计学意义(F=1.047,均P>0.05)。ACS组血清CRP水平(12.07±15.83)mg/ml与SA组(1.58±1.19)mg/ml、对照组(1.43±1.10)mg/ml相比差异有统计学意义(F=3.854,P<0.05)。CD40与CRP之间呈显著相关性(r=0.406,P<0.05)。结论ACS患者外周血单个核细胞CD40表达增高,可能是冠状动脉粥样硬化病变活动性的血清学标志物。

Objective To investigate the relationship of CD40 and CD40L on peripheral blood mononuclear cells with acute coronary syndromes （ACS）. Methods The expression of CD40 and CD40L on peripheral blood mononuclear cells was detected by indirect immunofluorescence flow cytometry and the circulating levels of C-reactive protein （CRP） were determined by enzyme linked immunosorbent assay （ELISA） in 23 patients with acute coronary syndromes, 16 patients with stable angina, and 15 controls. Results CD40 levels were significantly higher in patients with acute coronary syndromes than those in patients with stable angina and controls （P〈0.05）. There were no significant differences in CD40L levels among three groups （P〉0.05）. CRP levels were significantly higher in patients with acute coronary syndromes than those in patients with stable angina and controls （P〈0.05）. CD40 levels were correlated with serum CRP levels （r=0.406, P〈0.05）. Conclusion The expression of CD40 on peripheral blood mononuclear cells is significantly elevated in acute coronary syndromes. CD40 may be a new candidate marker for activity of coronary disease.