HPLC法测定抗炎退热片中黄芩苷的含量

Determination of Baicalin in Kangyan Tuire Tablets by HPLC

目的:建立高效液相色谱法测定抗炎退热片中黄芩苷的含量。方法:采用高效液相色谱(HPLC)法,以C18柱(25mm×4.6mm;(?)5μm)为色谱柱;以甲醇-0.1%磷酸溶液(50:50)为流动相;检测波长为280nm;流速为1.0ml/min。结果:黄芩苷进样量在6～296μg/ml范围内与峰面积线性关系良好(r=0.9999),平均回收率为99.35%,RSD=0.794%(n=6)。结论:HPLC法快速、准确、重现性好,可用于抗炎退热片的质量控制。

Objective： To establish a HPLC method for the content determination of baicalin in Kangyan Tuire Tablets. Methods： HPLC method was adopted and a C18 column（250mm× 4.6mm; φ5μm ）was used.The mobile phase was methanol-0.1% Phosphoric acid（ 50： 50）. The detection wavelength was at 280nm. The flow rate was 1.0ml/min. Results： The calibration curve of baicalin was linear in the ranger of 6-296μg/ml（r = 0.9999）, the average of recovery was 99.35 %, RSD= 0.794 % （n = 6）. Conclusion： The method is quick and accurate with good repeatability and can be used for quality control Kangyan Tuire Tablets.