逆胶束介导化学发光法测定烟酸占替诺

Chemiluminescence Analysis of Xanthinol Nicotinate in Reversed Micelle Media

在酸性条件下，烟酸占替诺分子中氮原子被质子化后与阴离子AuCl4^-形成离子缔合物，该缔合物被三氯甲烷带人鲁米诺的氯化十六烷基三甲基铵（CTAC）逆胶束中，离解出来的AuCl4^-立即与鲁米诺产生化学发光。在一定浓度范围内，发光强度与烟酸占替诺的含量呈线性关系。在优化的试验条件下，烟酸占替诺的检测线性范围为0．01—15×10^-6G/mL，检出限（3σ）为0．4×10^-9g/mL，对浓度为1．0×10^-6g/mL的烟酸占替诺进行11次平行测定，其RSD为1．26％。方法简单、灵敏，已成功用于针剂中烟酸占替诺的测定。

Under acidic condition, the nitrogen in xanthinol nicotinate was protonated followed by its reaction with AuCl4^- anion to form an ion-complex, which was carried into a reversed micelle of cetyltrimethylammonium chlorine containing luminol by chloroform, and the dissociated AuCl4^- reacted with luminol to produce an analytical chemiluminescence signal. In a certain concetration range, the CL intensity was proportional to the concentration of xanthinol nicotinate. Under the optimum conditions, the linear range was 0. 01 to 15 × 10^-6 g/mL, and the limit of detection was 0. 4 × 10^-9 g/mL with a relative standard deviation（ n = 11 ） of ca. 1.26% for xanthinol nicotinate of 1.0 × 10^-6 g/mL. The method is simple, sensitive and has been applied to determining the studied drug in injection successfully.