二安替比林甲烷共振瑞利散射法测定硫酸葡聚糖钠

Resonance Rayleigh Scattering Method for the Determination of Dextran Sulfate Sodium with Diantipyrylmethane

采用共振瑞利散射法研究了二安替比林甲烷与硫酸葡聚糖钠的相互作用及其分析应用.在pH为10.0的Britton-Rob- inson缓冲溶液中,二安替比林甲烷与硫酸葡聚糖钠结合后共振瑞利散射急剧增强,最大散射峰在352nm处.研究了此反应体系的最佳实验条件以及共振瑞利散射光谱的特征,在最佳条件下,共振瑞利散射信号的增强与硫酸葡聚糖钠的浓度在0.05～1.5μg·mL^(-1)范围内成正比·检出限为17.0ng·mL^(-1),该法用于痕量硫酸葡聚糖钠的测定,灵敏,简便.

A new method for the determination of dextran sulfate sodium （DSS） with diantipyrylmethane （DAM） by resonance Rayleigh scattering （RRS） technique has been proposed, In pH 10.0 Britton-Robinson buffer medium, the interaction of DSS with DAM occurred, which greatly enhanced the RRS intensity with a maximum scattering peak at 352 nm. The interaction conditions were optimized. The affecting factors and characteristics of RRS for the interaction of DSS with DAM were investigated. Under the optimum condition, the enhancement of the RRS signal is directly proportional to the concentration of DSS in the range of 5.0× 10^-2 --1.5 μg · mL^-1 and its detection limit is 17.0 ng·mL^-1.