脑损伤患者血清肌酸激酶及乳酸脱氢酶同工酶的分析与临床应用

The Clinical Application of Serum CK and LDH Isoenzyme for Severe Head Injury

为探讨肌酸激酶(CK)同工酶及乳酸脱氢酶(LDH)同工酶在脑损伤疾病诊断中的应用,使用日立7060全自动生化分析仪,酶偶联法检测肌酸激酶,速率法测乳酸脱氢酶,法国SEBIA全自动电泳仪琼脂糖凝胶电泳法测定79例脑损伤性疾病患者的肌酸激酶同工酶、乳酸脱氢酶及同工酶.研究发现:脑损伤后5天内,患者血清CK及LDH总活力显著升高(p<0.05);CK—MB、CK—BB随着病情的好转而降低直至恢复正常;LDH同工酶LDH_1、LDH_2、LDH_3、LDH_5在第一天和第二天有显著性变化,24h内LDH_1、LDH_2、LDH_3显著下降(p<0.05),LDH_5显著升高(p<0.05).血清CK及同工酶、LDH及其同工酶的测定对脑损伤患者的诊断和预后判断均有重要意义.

Objective To study the application of CK isoenzyme and LDH isoenzyme in diagnosing the head injury. Methods： Using Hitachi7060 automatic analyzer and enzyme-speed method to detect CK and LDH respectively, and the agarose gel in France SEBIA automatic electrophoresis system is adopted to analyze CK isoenzyme and LDH isoenzyme. Results： Compared with normal, the serum CK and LDH has significantly increased for head injury patient （p〈0. 05）, CK-MB and CK-BB decreased as conditions improved and lower to normal, LDH1 ,LDH2 ,LDH3 decreased （p〈0.05） and LDH5 increased（p〈0.05） significantly in 24 hours . Conclusions： The detection of serum CK and LDH isoenzyme has great significance in head injury diagnosis and treatment.