水分胁迫下豌豆保护酶活力变化及脯氨酸积累在其抗旱中的作用

Water Stress Induced Changes in Protective Enzyme Activities and Effects of Proline Enhancement on Drought Resistance in Pea

对不同抗旱力豌豆水分胁迫下叶片相对含水量（ＲＷＣ）、质膜相对透性、超氧化物歧化酶（ＳＯＤ）、过氧化氢酶（ＣＡＴ）、过氧化物酶（ＰＯＤ）活力及脯氨酸含量进行了测定，研究了豌豆抗旱的生理基础。结果表明：水分胁迫下不同抗旱力豌豆细胞质膜相对透性增加，叶ＲＷＣ下降，ＣＡＴ和ＰＯＤ活力上升。抗旱性强品种ＳＯＤ活力上升，抗旱性弱品种ＳＯＤ活力下降，脯氨酸积累。复水后细胞质膜相对透性下降，ＲＷＣ上升，ＣＡＴ和ＰＯＤ活力下降。水分胁迫下，抗旱性强品种抗脱水力强，其保护酶对缺水的快速反应以及保护酶间协同作用分解氧自由基防止膜脂过氧化，增强膜的柔韧性及脯氨酸的渗透调节作用是抗旱的主要生理原因。

hanges in relative water content(RWC),membrane permeability and activities of superoxide (SOD),catalase(CAT) and peroxidase (POD),and proline content were measured in four cultivars with different drought resistance growing under water stress conditions.The results showed that RWC decreased,membrane permeability and activities of CAT and POD increased,SOD activities arised in drought resistant cultivars and declined in drought susceptible ones.Proline content increased in all cultivars tested.After the plants recovered from water stress it was found that RWC was up,membrane permeability and activities of SOD,CAT and POD were all down.These suggested that cultivar with higher drought resistance was more on stable RWC and its protective enzyme in response to water deficit was faster and more proline accumulated than cultivars with less drought resistance.