金雀异黄素对外伤性增生性玻璃体视网膜病变中bFGF和PCNA表达的抑制

Inhibitory Effects of Genistein on the Expression of bFGF and PCNA in Traumatic Proliferative Vitreoretinopathy

目的探讨金雀异黄素对兔外伤性增生性玻璃体视网膜病变(proliferative vitreoretinopathy,PVR)视网膜组织碱性成纤维细胞生长因子(basic fibroblast growth factor,bFGF)mRNA和增殖细胞核抗原(proliferating cell nu-clear antigen,PCNA)表达的影响。方法建立外伤性PVR兔模型,分为模型对照组及金雀异黄素治疗组。每组于制模后7、14、21、28d分别将2只模型兔眼球取出制作标本,采用原位杂交法检测视网膜组织bFGF mRNA表达,采用免疫组织化学法检测PCNA表达。结果①外伤性PVR视网膜组织bFGF mRNA和PCNA表达呈现先升高后降低的动态变化,14d时达到峰值;②金雀异黄素治疗组在7、14、21、28d时视网膜组织bFGFmRNA和PCNA表达均明显低于模型对照组,差异均有显著性意义(均P<0.05)。结论金雀异黄素能抑制外伤性PVR视网膜组织bFGF mRNA和PCNA表达,具有防治外伤性PVR的潜能。

Objective To study the effects of genistein on the expression of bFGF and PCNA in traumatic proliferative vitreoretinopathy （PVR） model. Methods Traumatic PVR models were induced in pigmented rabbits. The models were divided into 2 groups： group A （negative control）, group B （genistein 40 μg）. In each group, 2 eyes were enucleated respectively on the 7th, 14th, 21st, 28th day after surgery. The expression of bFGF mRNA in retina was detected by in situ hybridization examination, and the expression of PCNA in retina by immunohistochemistry. Results In group A, the expression of bFGF mRNA and PCNA protein was up-regulated at first and then down-regulated, with the peak value at 14th day. In group B, the expression of bFGF mRNA and PCNA protein was obviously down-regulated as compared with group A at 7th, 14th, 21st and 28th day （all P〈0.05）. Conclusion Genistein can significantly inhibit the expression of bFGF mRNA and PCNA protein in traumatic PVR, suggesting the potential of genistein preventing and treating traumatic PVR.