制备甲壳素过程中EDTA脱钙法的研究

Study on the Preparation of Chitin with EDTA Decalcify Method

通过对不同条件下制备而成的甲壳素进行灰分(含钙量)、红外光谱、扫描电镜等分析测试,比较了采用EDTA溶液代替盐酸制备甲壳素的脱钙效果以及甲壳素在结构、性能参数等方面的变化。其结果为:无论是用pH=4,还是pH=10的EDTA饱和溶液,其脱钙效果都远远大于pH=4的盐酸溶液;由不同脱钙法所制得的甲壳素其结构基本一致,但相对分子质量有较大差别,其中以EDTA脱钙法制备的甲壳素相对分子质量较大。

The ash content （calcium content）, DD, IR as well as SEM was used to analyze the chitin prepared by different conditions. The results showed that the saturate solution of EDTA has better decalcification effect in chitin preparation than HCl. No matter the pH of EDTA solution is four or ten, their effects are all much better than that of HCl （pH=4）. Therefore EDTA may replace HCl for decalcification in chitin preparation. Although comparing with HC1, the cost of EDTA is high and the disposable investment is also large, it is very easy to be retrieved and could be used repeatedly. The DD of product slightly increased along with the increase of concentration of alkaline solution and the time of reaction.