组成型纤维素酶高产菌的筛选及产酶条件优化

Screening of an Efficient Constitutive-Producting Strain and Fermentation Optimization for Cellulase Production

从富含植物纤维素的土样中筛选得到一株组成型纤维素酶产生菌LC-9,结合Biolog微生物自动鉴定仪和16S rDNA序列分析,该菌株鉴定为Bacillus subtilis。通过发酵与产酶条件优化,确定了菌株最佳培养条件。在优化条件下发酵35 h后,内切纤维素酶活力可达1 301.4U/mL,木聚糖酶活力可达289.2 U/mL。该菌株产酶发酵周期短,无需纤维素类物质的诱导,推测为一株新型组成型多功能纤维素酶高产菌株。

An efficient strain LC-9 secreting was newly isolated from the soil. On the basis of the result of taxonomic studies including the analysis of Biolog Identification System and 16S rDNA sequence analysis, LC-9 was identified as Bacillus subtilis.According to the effect of various substance on the cell growth and activity of cellulase, the components of optimized medium and fermentation conditions were confirmed. Under optimized conditions, 1 301.4 U/mL of CMCase and 289.2 U/mL of Xylanase in cultured supernatant were obtained after 35 h fermentation. Without cellulose or xylan inducers were used during cellulase production.