多菌种混合发酵生脉饮药渣生产蛋白饲料工艺条件优化

Optimization of Process Conditions of Shengmaiyin Residues to Produce Protein Feed by Multi-strains

以生脉饮药渣为原料,利用康宁木霉(Tk)、产黄纤维单胞菌(Cf)、产朊假丝酵母(Cu)和黑曲霉(An)多菌种混合发酵生产蛋白饲料。通过单因素和正交试验对发酵条件进行优化,试验结果表明:先接种20%的Tk+Cf(比例2∶1),发酵2 d后再接种20%的Cu+An(比例1:1),在(NH4)2SO4添加量为5 g/dL、初始pH值为6、料水比为1∶2、温度为30℃的条件下发酵5 d,其发酵产物中真蛋白质量分数增加86.96%,粗纤维质量分数降低20.09%。

In this study, protein feed production from shengmaiyin residues through the mixed fermentation by multi-strains which included Trichoderma koningii （Tk）, Cellulomonas flavigena （Cf） ,Candida utilis （Cu） and Aspergillus niger （An）. The fermentation conditions were optimized by single factor and orthogonal experiment. The result showed that, during the first 2 days, inoculating Tk＋Cf of volume 20G（proportion 2 ： 1） and then inoculating Cu＋An in volume 20G（proportion 1： 1） after 2 days. 5% （NH4）2SO4, intitial pH 6, the ratio of material to water was 1 ： 2, temperature 30 ℃ and fermentation time was 5 d. It was found that the real protein content increased by 86.96%, crude fiber decreased by 20.09% compared with that of the control.