小鼠对口腔念珠菌感染的体液免疫应答

The humoral immune response to oral candidiasis in two strains of inbred mice

目的建立敏感株和阻抗株小鼠口腔念珠菌感染模型,揭示两种小鼠对口腔念珠菌感染的体液免疫反应。方法雌性BALB/c和CBA/CaH小鼠及白色念珠菌3630用于本研究。1×108念珠菌/20!l接种于小鼠的口腔黏膜,建立口腔念珠菌感染模型;SDS-PAGE和Western-blotting用于检测血清抗体。结果念珠菌敏感株鼠CBA/CaH的口腔感染比阻抗株鼠BALB/c严重(P<0.05);CBA/CaH小鼠在口腔免疫后5周产生了IgG1、IgG2a、IgM,而BALB/c小鼠仅产生IgG1。结论念珠菌敏感鼠CBA/CaH的口腔念珠菌感染比念珠菌阻抗鼠BALB/c感染更为严重;抗白色念珠菌抗体IgM可能对宿主的口腔念珠菌感染起保护作用。

Objective The aims of this study were to compare the oral infection with C.albicans in two inbred mice which are genetically susceptible or resistant to candidiasis using a murine model, and to explore the patterns of antibody production from these two mice after oral challenge with C.albicans, Methods Inbred female BALB/c and CBA/CaH mice and C.albicans 3630 were used in this study. Oral infection was established on murine mouth by inoculation of live C.albicans, and monitored by swabbing. The antibody production profile of mouse was determined with Western Blotting after oral immunization with yeasts. Results CBA/CaH mice demonstrated a severer oral infection and a stronger humoral response the infection by producing IgG1, IgG2a and IgM than BALB/c mice. Conclusions Oral infection pattern in this study is consistent with the pattern of systemic infection for these two strains of inbred mice, confirming that the genetic factors play a key role for severity of both oral and systemic candidiasis. However, C.albicans-specific IgM might play a protective role for oral Candida infection in mice.