乙型肝炎病毒在卵巢组织中的表达、定位及临床意义

Expression and location & clinical significance in ovarian tissues from patients with HBV infection

目的通过对乙型肝炎病毒(HBV)感染者卵巢组织中HBsAg、HBcAg和HBVDNA的检测来观察HBV在卵巢组织中表达与定位情况。方法对26例血清学HBV标志阳性的卵巢肿瘤患者用免疫组化方法检测卵巢活检组织HBsAg、HBcAg的表达;应用PCR技术对免疫组化阳性病例进一步检测卵巢组织HBVDNA。结果26例卵巢组织中HBsAg阳性9例,HBcAg阳性9例,阳性率均为34.6%。总阳性率为46.2%(12/26);其中HBsAg和HBcAg同时阳性6人,占23.1%。阳性信号呈棕褐色细颗粒状弥漫分布。HBV血清学标志多项阳性者卵巢组织中HBsAg、HBcAg的检出率与HBV血清学标志单项阳性者差异无显著性。12例免疫组化阳性患者检出卵巢HBVDNA的有7例,阳性率为58.3%。结论HBV感染者卵巢组织中可检出HB-sAg、HBcAg和HBVDNA,卵巢组织中HBsAg、HBcAg受血清HBV的变化影响小,与肝炎病变不呈平行关系。说明HBV可以感染与定位到卵巢组织,卵巢组织中检出HBV丰富和扩充了经典的母婴传播机制。

[Objective] To investigate the presence of HBsAg,HBeAg and HBV DNA in ovarian tissues from patients with HBV infection. [Methods] HBsAg and HBeAg was examined in ovarian biopsy tissues from 26 patients with HBV infection by immunoeytoehemistry, and HBV DNA was detected in ovarian tissues by PCR. [Results] HBsAg and HBeAg were present with the same positive rate of 34.6% （9/26）. The total positive rate was 46.2% （12/26）. Of the 26 cases, both HBsAg and HBeAg were positive in 6（23.1%） cases. Brown positive particles were diffusely distributed in ovarian cells. The positive rate of HBV DNA was 58.3% （7/12）. [Conclusion] HBsAg,HBeAg and HBV DNA can be detected in ovarian tissues from patients with HBV infection. The presence of HBsAg and HBeAg in ovarian tissues did not correlated with the HBV markers in serum. The detection of HBV in ovarian tissues enriched and expanded the classical mechanism of vertical transmission.