摘要
在β-环糊精6位羟基上导入苄氧羰基(CBZ)保护苯丙氨酸,并将该衍生物与硅胶键合制备了高效液相色谱手性固定相.采用莫氏颜色试验、红外光谱、X射线光电子能谱等方法表征了环糊精衍生物和键合固定相.以苯和萘为测试物评价了键合固定相色谱柱的效能.使用该色谱柱实现了硝基苯酚和氨基苯酚位置异构体的分离.盐酸克伦特罗和5,6-氧-异丙叉基-3-硫-己酸-1,4-内酯的手性异构体也获得一定程度的拆分.
CBZ-phenylalanine was reacted with 6-OH of β-cyclodextrin, then the derivative was bonded to silica to produce a new chiral stationary phase （CSP） for high performance liquid chromatography（HPLC）. The derivative of β-cyclodextrin and the CSP were characterized by Molisch color reaction, IR and XPS. Chromatographic performance of the bonded silica CSP was evaluated using benzene and naphthalene as testing materials. The m-, o-, p-isomers of nitrophenol and aminophenol were separated well on the column of the CSP. Enantiomers of clenbuterol hydrochloride and 5,6-O-isopropyl-3-thio-hexanoic acid-1, 4-1actone were investigated and tolerably separated on the column.
出处
《北京理工大学学报》
EI
CAS
CSCD
北大核心
2008年第8期749-752,共4页
Transactions of Beijing Institute of Technology
基金
北京理工大学基础研究基金资助项目(20070642009)
