摘要
本文探讨利用交变脉冲电场凝胶电泳技术，分离、分析稻瘟病菌染色体ＤＮＡ的适宜条件。结果认为，利用ＰｒｏｎａｓｅＥ或国产试剂蛋白酶可以代替通常使用的蛋白酶Ｋ，用于染色体去蛋白质。在固定的电场设置下，样品的分离效果与电场强度、脉冲时间、电泳时间和电泳系统的温度有关；对分离效果影响最大的是电场强度与脉冲时间。实验初步得出稻瘟病菌染色体ＤＮＡ的电泳条件为，有效脉冲时间０．５～１１０ｓ，大多数分子集中在有效脉冲时间９～７０ｓ范围。
In the paper, the suitable condition for separating and analysing chromosomes DNA of Pyricularia grisea were researched by pulsed field gel electrophoresis The results showed that proteinase E or reagent proteinase made in China can replace proteinase K In a certain electric field installation, the separated effect related to voltage, pulse time, electrophoresis time and temperature of electrophoresis system, among which voltage and pulse time were the main factor It was tested that the effective pulse time 0 5-110 sec was the electrophoresis conditions of the fungus chromosomes DNA
基金
福建省科委自然科学基金
关键词
水稻
稻瘟病菌
染色体
电泳
Rice
Pyricularia grisea
Chromosome electrophoresis
