摘要
樱桃带化病是从以色列引种樱桃园中发现的新病害。本文报道了按照检测植物菌原体（ｐｈｙｔｏｐｌａｓｍａ）的方法提取ＤＮＡ，扩增患病植株中植物菌原体的１６ＳｒＤＮＡ片段，证明樱桃带化病中有植物菌原体存在，并对此扩增片段进行限制性酶切片段长度多态性（ＲＦＬＰ）分析。根据ＲＦＬＰ分析结果，参照Ｌｅｅ，Ｉ．Ｍ．等的文献资料，对本次樱桃带化病病原进行鉴定与分类，初步定为Ⅶ类。
Cherry fasciated disease is a disease which was found in the cherry sapling imported from Israel.DNA amplification by PCR was used to detect the phytoplasma associated with cherry fasciated disease.The 16 S rDNA sequence amplified was analyzed by restriction endonuclease digestion.The RFLP pattern(sum of results analyzed by 7 restriction enzymes)was compared with the results of other phytoplasma previously described by Lee,I.M.et al.We propose that cherry fasciated disease should belong to Group Ⅶ.
出处
《林业科学研究》
CSCD
北大核心
1997年第5期478-481,共4页
Forest Research
基金
国家自然科学基金
